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Further screening of a gRNA multiplex library identified the unanticipated combination of knockdown of PYC1 (pyruvate carboxylase) and NTH2 (trehalose) which resulted in a 3-fold increase in titer of 227
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They work together in a synergistic loop to neutralize free radicals and protect cell membranes
Cycling Details Loading (Weeks 1-4): 10 units 5x/week (weekdays on, weekends off)
