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Eur J Med Res 30 , 248 (2025)

4-hydroxy-2(E)-Nonenal Facilitates NMDA-Induced Neurotoxicity via Triggering Mitochondrial Permeability Transition Pore Opening and Mitochondrial Calcium Overload

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J Endocrine Soc

subsequently, glutamate will convert to -ketoglutarate (-KG) as the main component of Tricarboxylic Acid cycle (TCA) by mitochondrial glutamate dehydrogenases (GLUD), -KG mainly incorporates into TCA cycles to assist the production of NADPH and nucleotide synthesis

After labeling, cells were gently quenched, washed three times with DPBS, fixed and permeabilized with ice-cold methanol for 10 min