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In darkness, the SCNs inhibitory effect is lifted

The animals were put into 2 groups of 6 (Group 1 and Group 2)

[PMC free article: PMC6500795] [PubMed: 31069279] 130

Animal experiments were subject to ethical review by the Francis Crick Animal Welfare and Ethical Review Body and carried out under UK Home Office project license P319AE968 or by the CRUK Beatson Institute (reviewed and approved by the University of Glasgow and UK Home Office) and carried out under UK Home Office project license 70/8645

Insoluble proteins were discarded by high-speed centrifugation at 2000 g for 10 min at 4 C