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The extracted samples were analyzed using the Ultimate 3000 nano-UPLC system (Thermo Scientific) and Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific)

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[DOI] [PubMed] [Google Scholar] Bailey SM, Cunningham CC

Dugan CM, MacDonald AE, Roth RA, Ganey PE

Secondly, the refolding process necessitates high concentrations of denaturants (e.g., urea, guanidine hydrochloride) and other reagents 22 , and its efficiency often decreases with increasing protein concentration, limiting scalability for large-scale production 23

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