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HUVEC and HDMEC were maintained in Endothelial Cell Basal Medium (PromoCell) supplemented with 2% FCS, 0.4% endothelial cell growth supplement, 0.1 ng ml 1 recombinant human EGF (rh EGF), 1 ng ml 1 recombinant human basic fibroblast growth factor, 90 g ml 1 heparin and 1 g ml 1 hydrocortisone

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Cytokines are small but powerful biologically active proteins that act as signaling molecules within the immune system coordinating the bodys response to infection, regulating inflammation, and managing normal cellular growth

H., Zhou Y., et al

doi: 10.1038/nature12000

Even though these otoprotective agents represent a promising new therapeutic strategy for ameliorating, delaying, or even preventing the impact of noise on hearing, finding the ideal therapeutic agent will remain a challenging topic for the future