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Indeed, results showing that such ablation reduces or obliterates the endothelial responses to shear provide evidence that the glycocalyx functions as a mechanosensor or mechanotransducer

The fragment of interest was PCR amplified by using specific primers comprising the KpnI and BglII restriction enzyme sites as listed in Appendix Table S2

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If you need to transfer to new containers for aliquoting, use sterile borosilicate glass vials with rubber stoppers

Svardal et al