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In particular, GSDME-deficiency significantly attenuated the hippocampal atrophy and changed levels of DAM markers in GCLC-KO mice

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

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This review provides insights into the mechanisms through which amino acids contribute to stress mitigation and discusses practical implications for sustainable aquaculture, aiding researchers, feed manufacturers, and fish farmers in formulating functional diets to improve fish resilience and productivity.