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RNA sequencing Total RNA was extracted from adipose tissue using the QIAzol Lysis Reagent (#79306, Qiagen Venlo, Netherlands) and from liver using the Nucleospin RNA kit (#740955, Macherey-Nagel GmbH & Co, Dren, Germany)

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De-acetylation of Prx3 at Lys92 by the de-acetylase sirtuin 4 confers protection against ferroptosis in the context of liver ischemia-reperfusion injury [190]