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ROS level in MM cells was measured by detecting the fluorescence intensity at ex = 490 nm/ em = 525 nm
Dithiothreitol (60 mmol/L in 0.1 mol sodium tetraborate/L) was added to convert oxidized glutathione (GSSG) to GSH and was then alkylated by adding iodoacetamide (0.5 mol/L) in 0.1 mol ammonium bicarbonate/L
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The P3 side chain is solvent exposed, and the corresponding S3 subsite also shows tolerance to a wide range of functional groups
