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Western blot analysis and immunoprecipitation Proteins from the cell conditioned media of THP-1 cells were precipitated by the addition of 3 volumes of ice-cold acetone, incubation at 20 C for 18 hours and centrifugation at 13,000 g for 10 min at 4 C

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It is generally recommended to take it either 30 minutes before a meal or two hours after eating