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Steady-state kinetics were measured using a pyrophosphate assay for the apo and AdoCbl-bound dark/light states of the Ab DPcob protein, as well as the truncated DGC domain only (Fig

DISCUSSION The goal of this study was to develop an AngIV-derived molecule that retained the procognitive/antidementia activity of AngIV and Nle 1 -AngIV but possessed improved pharmacokinetic properties, thus allowing it to penetrate the BBB in sufficient quantities to reach therapeutic levels in the brain

Materials and Methods Chemicals and Materials Aroclor 1254-induced male Sprague-Dawley rat liver microsomes (RLM) (part no.: M10001, lot no.: QEB and OTS) were purchased from BioreclamationIVT (Baltimore, MD)

Analysis was conducted using an LC-MS QTRAP 6500+ system (SCIEX) equipped with a BEH C18 column (2.1 mm 10 cm, 1.7 m, Waters) and operated in ESI+ or ESI mode

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