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The clarified cell lysate was either directly submitted to further protein purification steps or flash-frozen in liquid nitrogen after addition of 10% glycerol and stored at 80 C for use in biocatalytic reactions

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As reported in the cases of PVY NTN and TuMV, the GSH form and total glutathione levels could be upregulated in susceptible plants until the point of symptom occurrence and then highly depleted (Otulak-Kozie et al., 2022b, 2023)

Takaoka, Y

Novel nicotinamide analog as inhibitor of nicotinamide N-methyltransferase

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