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Given its energetic requirements, it should be no surprise that researchers are increasingly making connections between the brain and mitochondrial health (mitochondria are power horse organelles found in most cells and are responsible for cellular respiration and energy production)

The primary antibodies utilized in this study included DNA/RNA Damage (1:200 dilution, ab62623, Abcam, America), p-ATM (1:50 dilution, R380751, ZEN-BIOSCIENCE, China), p-ERK (1:50 dilution, R380698, ZEN-BIOSCIENCE, China), 8-oxoguanine (1:50 dilution, 130914, Santa Cruz Biotechnology, America), H2A.X (phospho S139) antibody (1:200 dilution, ab81299, Abcam, America), Paxillin antibody (1:200 dilution, ab32084, Abcam, America), The cytoskeleton was stained with FITC-conjugated phalloidin (1:200 dilution, A12379, Invitrogen, America)

7 Dosages used in the studies ranged from 400 to 2000 mg per day

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