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### AOD-9604 Mechanism of Action AOD-9604 is believed to promote **fat loss** through: - Stimulation of lipolysis in adipose tissue via beta-3 adrenergic receptor pathways - Inhibition of lipogenesis (new fat formation) - No effect on growth hormone receptors (does not promote tissue growth) - No effect on IGF-1 levels or blood glucose Importantly, AOD-9604 does **not** suppress appetite or affect food intake it is purely a lipolytic **fat loss peptide**

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Furthermore, PNPLA2 in the heart is required to maintain the supply of FAs for cardiac function during thermogenesis, as PNPLA2 cardiac-specific deficient mice are cold intolerant (102)