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Application of this reagent to the acetate of methyl N -Boc-4-hydroxyprolinate [(2 S ,4 R )- 79 ] gave the protected pyroglutamate 80 which was transformed into dimethyl N -Boc-4-hydroxyglutamate [(2 S ,4 R )- 81 ] (Scheme 20) [82]

, These studies demonstrated the successful detection of proteins by depositing analytes on a thin film of C60 fullerene (10 nm thick) and forming analyte ions through nitrogen laser irradiation, with successful identification of proteins such as cytochrome c (12 kDa) and BSA (66 kDa)

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NAD captureSeq indicates NAD as a bacterial cap for a subset of regulatory RNAs