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Treating cells with L-buthionine sulfoximine (BSO), an inhibitor that blocks the synthesis of GSH, renders neuronal SH-SY5Y cells expressing mutant valosin-containing protein (VCP) R487H more susceptible to oxidative stress (Hirano et al., 2015) and increases mutant SOD1 toxicity (Alvarez-Zaldiernas et al., 2016

It is important to preserve the quality and quantity of the samples for diagnosis [24]

Microvasc Res 75(2):144154 Funahashi Y et al (2010) Notch regulates the angiogenic response via induction of VEGFR-1

Lysates were deposit on Bolt SDS page gel (Invitrogen) and transferred on nitrocellulose membrane (ThermoFisher) using Trans Blot Turbo transfert (Biorad)

L-S, R-buthionine sulfoximine: historical development and clinical issues