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Briefly, cells are cultured with the STEMdiff TM definitive endoderm basal medium with supplements A and B for 24h followed by STEMdiff TM definitive endoderm basal medium with supplement B for 48h with daily medium change (Xia et al., 2017)

Because it lacks this fundamental human testing, the FDA considers it an experimental compound with unknown long-term risks, preventing it from being approved as a legal medical treatment

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