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After co-cultured for 12 h at 37 C, the macrophages on the lower surface of inserts were fixed with 4% formaldehyde for 30 min, stained with DAPI, and counted according to the previously established protocol [40]

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AX may influence Polyunsaturated fatty acid (PUFA) metabolism through the modulation of fatty acid composition and the prevention of oxidative degradation rather than by directly stimulating PUFA biosynthesis [81]

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Moreover, several bioactive compounds found in fish, such as n-3 polyunsaturated fatty acids, taurine, and other active substances, may contribute to the health benefits of seafood