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First, fragmentation and ligation of adapters were performed using the commercial MGIEasy Fast FS Library Prep Module (MGI, China) and MGIEasy UDB Adapter Kit (MGI, China) according to the manufacturers protocol

Nevertheless, TMAO has also positively affected diabetic peripheral neuropathy, glucose tolerance, and arterial hypertension (85)

GraphPad Prism 9 software (GraphPad Software, San Diego, CA, USA) was used for statistical analyses

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