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6) D 3044B1 AAAGGGATCCATGGTGTTTG (SEQ

9:E1211

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doi: 10.1097/00008469-200310000-00007

Materials and methods Cell culture The myeloma cell lines used in this study were ANBL-6 (a kind gift from Dr Diane Jelinek, Mayo Clinic, Rochester, MN, USA), INA-6 (a kind gift from Dr Martin Gramatzki, Erlangen, Germany 25 ), U266 (ATCC, Rockville, MD, USA), IH-1, 26 OH-2, 27 JJN3 (a kind gift from Dr Jennifer Ball, Department of Immunology, University of Birmingham, UK) and KJON (established in our laboratory)

aureus , 405-nm and 488-nm colinear lasers were set to 40 mW and 7090 mW for live/dead cell discrimination and GFP expression, respectively