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Retrieved 2016-03-02
Housekeeping probes to be used for normalization were selected based on the geNorm algorithm as implemented in the NormqPCR R library ( p -value) were calculated using the fast method as described in the nCounter Advanced Analysis 2.0 User Manual (NanoString)

M., Thanassi, J
Management of dyschromias in ethnic skin

Fluorescence intensity was analyzed using ImageJ (v1.54 f) software (National Institutes of Health), with the regions of interest (ROIs) selected using the threshold function

For individuals where pigmentation has a metabolic or oxidative origin (rather than a purely superficial one), this makes a meaningful difference
