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Metabolic regulation of chromatin modifications and gene expression

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Micro slide 8-well glass bottom plates (Catalog 80807, Ibidi, Grfelfing, Germany) were treated with 40 L of a 1 mg/ml Concanavalin A (Catalog C2010, Sigma Aldrich, prepared in 10 mM Sodium phosphate buffer pH 6, 10 mM CaCl 2 ,1 mM MnCl 2 , 0.01% NaN 3 ,) solution, incubated for 5 min and then washed twice with 40 L of fresh medium