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In the second scenario, the final yield of your elution is close to 100 mg of GST-fusion protein, so youre feeling confident that you used the right binding buffer and that your protein isnt too big
If your B12 level is below 200 pg/mL (picograms per milliliter), you're considered to have a deficiency

Table 1 shows the baseline results of the non-responder and responder groups

The individual study effect sizes were then entered into a random effects meta-analytic model using restricted maximum likelihood estimation
It is produced in the liver, kidneys, and pancreas, and stored mainly in skeletal muscle