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10.3389/fimmu.2018.02009 71 CruzJ

The mixture was freeze-thawed twice at 20C for 1 h and 37C for 15 min, the supernatant was obtained by centrifugation at 12000 rpm for 10 min as the whole cell extract
Claudin-1, -2 and -3 are selectively expressed in the epithelia of the choroid plexus of the mouse from early development and into adulthood while claudin-5 is restricted to endothelial cells

doi: 10.3390/cosmetics12040139

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