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Mitochondrial biogenesis Mitochondrial biogenesis was first found when the level of intracellular mitochondrial content was significantly increased during endurance training in the study of Holloszy et al

PPAT transfers glutamine-derived nitrogen to 5-phosphoribosyl pyrophosphate (PRPP), and this step is considered the initial step in purine biosynthesis 37

Analysis of the structures of CapD and HsGGT suggested that the putative acceptor binding site is proximal to the donor binding site, located in the deep groove, and is highly variable and flexible as compared to the conserved and rigid donor binding site

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Together, these results suggested that this phenotype could be exploited as cancer therapy through the use of inhibitors of glutaminolytic enzymes or treatment which induce glutamine depletion like L-asparaginase