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Nevertheless, CS-induced alterations in glial cell profiles have been linked to cognitive impairment in animal models, reinforcing the mechanistic connection between pulmonary inflammation, systemic spill-over, and neuroinflammatory outcomes [19]

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These receptors are coupled to a chloride current
The cell lysates were solubilized in RIPA buffer containing 1 mM sodium orthovanadate, 1 mM sodium fluoride, 10 mM N-ethylmaleimide, 5 g/mL leupeptin, 5 g/mL aprotinin, and 2 mM phenylmethylsulphonyl fluoride, after which they were immunoprecipitated with FLAG beads

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