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Austin, M

Behery, O., Siston, R

10 March 2023

Results & discussion Method validation The HPLC method was validated as to specificity, linearity, sensitivity, accuracy, precision (repeatability and reproducibility), LOD, LOQ, and stability as per the ICH [50]ICH Q2 (R1), Validation of analytical procedures: text and methodology, in Proceedings of the International Conference on Harmonization, Geneva, Switzerland, 2005

To optimize SeNPs concentration for subsequent semen cryopreservation, three semen samples were individually split into four equals and diluted to a final concentration of 6.0 10 7 spermatozoa/ml with the basic extender supplemented with 0 g/ml SeNPs, 1 g/ml SeNPs, 2 g/ml SeNPs, and 4 g/ml SeNPs

& Boland, W