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Membrane was incubated at room temperature in blocking solution (1% bovine serum albumin, 1% goat serum in 1PBS) for 1 h, followed by incubation in blocking solution containing appropriate dilution of primary antibody for growth hormone receptor (R&D System, MN, USA) or phospho-Jak2 (Tyr1007/1008, Cell Signaling Technology, Inc

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