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KAT IV Better known as the mitochondrial glutamate-oxaloacetate aminotransferase 2 (GOT2), KAT IV (Uniprot P00505) has 430 amino acids and catalyzes the reaction of 2-oxoglutarate and L-aspartate to L-glutamate and oxaloacetate, playing an essential role in the malate-aspartate shuttle in mitochondria and in the synthesis of glutamate [210]

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In brief, the sections were incubated for 90 min at 25 C in blocking solution (10% horse serum in PBS), and then overnight at 4 C with primary antibodies: mouse anti-glial fibrillary acidic protein (GFAP) antibody (1:500, cat# MABN92, Sigma-Aldrich, USA) or mouse anti-ionized calcium binding adaptor molecule 1 (Iba1) antibody (1:500, cat# MABN92, Sigma-Aldrich, USA)

Chen K, Meric-Bernstam F, Zhao H, Zhang Q, Ezzeddine N, Tang LY, Qi Y, Mao Y, Chen T, Chong Z, Zhou W, Zheng X, Johnson A, Aldape KD, Routbort MJ, Luthra R, Kopetz S , Davies MA, de Groot J, Moulder S, Vinod R, Farhangfar CJ, Shaw KM, Mendelsohn J, Mills GB, Eterovic AK

Supporting information S1 Fig