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The membrane was first blocked in 5% (wt./vol.) skim milk powder (Lab M Limited, Heywood, UK) in tris-buffered saline with 0.1% tween20 (TBST) for 1 h at RT, then was probed with the primary antibody (concentrations and manufacturers in Supplementary Table 4) diluted in TBST with 5% BSA for 16 h at 4 C on a rocker

doi: 10.1056/NEJM200102223440802

BBA-Gen Subjects 544(3):496503

doi: 10.1056/NEJMc1713444 172 ValeroCLeeMHoenDZehirABergerMFSeshanVEet al
10.1093/jexbot/53.372.1367 13 BunkelmannJ
Dull or brown coloration may suggest nutrient degradation from heat exposure or age
