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measured the urinary HS concentration, which may reflect renal glycocalyx degradation, and found that HS was the only glycosaminoglycan that was significantly associated with mortality (hyaluronic acid and chondroitin sulfate were not) and that patients who died of sepsis had significantly higher mean HA concentrations at the start of the study than survivors (112)
The supernatant (serum) was carefully collected and stored at 80C until analysis
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