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So for me, the challenges are a little bit more

The financial cost of a new vial is minuscule compared to the cost of tainted data and wasted time

Knig, Phytochemistry 1996 , 44 , 471-474

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For the amplicon PCR, a 25 L reaction containing cDNA, 0.5 L of Platinum SuperFi II DNA Polymerase, 1 SuperFi II Buffer, and 500 nM forward and reverse primers was first heated to 98 C for 30 s, followed by cycles of 98 C for 10 s, 64 C for 10 s, and 72 C for 20 s

this advanced processing method was subsequently patented in 2014 under the trade name Eurosil 85 [2022]
