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There were 13 preclinical trials conducted on animal models of PD, of which ( n = 5) were conducted on the Drosophila melanogaster fruit fly model [2426, 28, 29], ( n = 4) on the Sprague-Dawley rat model [3336], ( n = 3) on the Swiss albino rat model [27, 30, 31], and one ( n = 1) was conducted on the monkey [32]

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In vitro characterisation of NAPstar sensors In vitro assays were performed in 50 mM Tris-HCl, 100 mM NaCl, 0.5 mM MgCl 2 at pH 7.5, except for pH titration and enzyme-coupled assays experiments