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Cytotoxicity assay Free LC, NLC-LC, and blank NLCs were tested for cytotoxicity on human adipose tissue-derived mesenchymal stem cells (MSCs) using the MTT assay 25

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This temperature was selected because sperm-washing media and related ART laboratory media are routinely stored at 4 C prior to use

extend to a broad range of applications including drugs, food, and plasma (Chen et al., 2016)

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