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Enhance the healing of chronic wounds and surgical incisions

Cassio Lynm from Amino Creative (Figure credit: Amino Creative, LLC, licensed under a CC-BY-NC-ND 4.0 International License [email protected]) was the medical illustrator for Figure 1 and Figure 2 (and support by Enlivity to create these figures was much appreciated)

10.1042/BJ20040298 Biochem

Hepatocyte-like cell maturation and maintenance For small molecule HLC maturation, hepatoblasts were incubated in maturation medium containing L-15 Leibovitz medium (Thermo Fisher Scientific), 100 mM Dihexa (DC Chemicals), 3% Insulin-Transferrin-Selenium supplement (Thermo Fisher Scientific), 10% Fetal Bovine Serum (Thermo Fischer Scientific), 2 mM GlutaMAX (Thermo Fisher Scientific), 10% Tryptose Phosphate Broth (Thermo Fisher Scientific), 10 M hydrocortisone-21-hemisuccinate (Sigma), 100 nM Dexamethasone (Sigma) and 50 g/mL Sodium-L-Ascorbate (Sigma)

This overgrowth leads to fermentation, gas production, inflammation, and symptoms that closely mirror IBS
