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The column was washed with lysis buffer (5 mM imodazole in lysis buffer) and increasing concentrations of NaCl (0.5 mM and 1 mM) followed by increasing concentrations of imidazole (5 mM and 20 mM, in lysis buffer) to remove contaminating proteins

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If the injury is resolving well, most patients complete the full cycle and then take a two to four week break before evaluating whether a second cycle is needed

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