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GPx4 uses GSH as a reducing substrate and converts it into oxidized form, also referred to as glutathione disulphide (GS-SG) ( NFE2L2 gene) (Habib et al., 2015), the xCT mRNA can be positively regulated by the activation of transcription factor 4 (ATF4) under oxidative stress (Sato et al., 2004), while its negative regulation by p53 results in cysteine deprivation and increased susceptibility to ferroptosis (Jiang et al., 2015)

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For compounds requiring acetic acid acidification for solubility and including some hydrophobic peptides , Bacteriostatic Water may need to be acidified with sterile acetic acid to achieve the required pH for dissolution before addition to the lyophilised compound

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The (His) 6 -tag was removed by overnight thrombin cleavage, followed by polishing with size-exclusion chromatography (SEC) using a Superdex 75 HiLoad 16/600 column (Cytiva, MA, USA) equilibrated in 20 mM Tris-HCl pH 8.0, and 150 mM NaCl