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Choline may raise blood levels of TMAO, a compound that may advance atherosclerosis (hardening of the arteries) by reducing the normal clearing of cholesterol and may increase plaque instability (which can cause it to become dislodged) (Koay, Cardiovasc Res 2020), although there do not appear to be studies assessing the link between citicoline (the form of choline in Cognizin ) and TMAO

A list of candidate biomarkers was determined from the proteins that presented a Welch t-test q- value less than 0.05 (after BenjaminiHochberg correction for multiple testing) and from the top contributors in component 1 and 2 of a sparse partial least square discriminant analysis (sPLS-Da) computed with the Mixomics R package 56

Although GSH is oxidized to glutathione disulfide (GSSG), the enzyme glutathione reductase (GR) ensures the regeneration of GSH from GSSG with the help of nicotinamide adenine dinucleotide phosphate (NADPH) (Figure 4) [83]

In summary, tweaking the reaction conditions to find a window in which the AON can be modified but not degraded was crucial to allow for this AON functionalisation protocol

doi: 10.21037/jgo.2018.04.07 240 TrapaniKMBoghossianLJ

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