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A value of 100% for the predefined critical effect sizes represents a two-fold change, which was also used in the present study to define a biologically relevant effect in the in vitro comet assay thus compensating for data variability and avoiding potentially false-positive results

It is important to note that B12 levels should ideally be measured before commencing replacement therapy, as supplementation rapidly normalises biochemical markers, potentially obscuring the diagnosis

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Martins RO, de Arajo GL, de Freitas CS, Silva AR, Simas RC, Vaz BG, Chaves AR (2021) Miniaturized sample preparation techniques and ambient mass spectrometry as approaches for food residue analysis