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Notably, supplementing NADPH and using a pharmacological inhibitor of aldose reductase an enzyme using NADPH oxidase for the formation of sorbitol in high-glucose conditions - successfully restored the sensitivity to LPS-induced NET formation in high glucose conditions, while reducing high glucose-induced cytosolic ROS production, neutrophil elastase secretion and spontaneous NET release (Joshi et al., 2020) (Table 4)

The nuclei were counter-stained with DAPI (Sigma-Aldrich)

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Metabolites produced by highly abundant organisms and/or highly expressed enzymes should more likely be present at quantifiable levels compared with the products of depleted organisms or minimally expressed enzymes