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The protein concentrations of the samples were determined using a Protein Assay Bicinchoninate Kit (Nacalai Tesque, Inc., Kyoto, Japan)

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(6) I Ct, Y Sakarya, N Kirichenko, et al (2016) Activation of the central melanocortin system chronically reduces body mass without the necessity of long-term caloric restriction Canadian Journal of Physiology & Pharmacology, 2016 Oct 19, Volume 95 (Issue 2), Pages 206214

They include various free radicals and reactive molecules formed from molecular oxygen and nitrogen, including superoxide anion radical (O ), hydroxyl radical (OH ), hydrogen peroxide (H 2 O 2 ), singlet oxygen ( 1 O 2 ), nitric oxide (NO), and peroxynitrite (ONOO ) [28]

Research efforts are also focused on understanding how variations in experimental design, concentration, and molecular stability may affect peptide behavior across different in vitro systems

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