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We generated 11 RP or MRD models by long-term mTORC1 inhibitor exposure either in vitro or in vivo

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In two hospital-based cohorts (studies A and B), fertility outcomes were assessed via clinical records, while in two other groups (studies C and D), women self-reported fertility issues

Some of these aldehydes such as 4-hydroxynonenal, 4-hydroxyhexenal, and malonaldehyde are highly reactive and may be considered as second toxic messengers which will cause the cross-linking polymerization of life macromolecules such as proteins and nucleic acids, and affect the activities of mitochondrial respiratory chain complexes and key enzymes in mitochondria, resulting in cell death (Esterbauer et al., 1991) (Figure 3)

The glutamate is then transported into the cell as gamma-glu-aa and is transformed into 5-oxoproline via gamma glutamyl cyclotransferase