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Usually 1 ml settled resin (2 ml 50% slurry) can bind 35-45mg GST-fusion protein (26 kDa)

Enzymatic Reaction Conditions The substrate solution used for the NsPCS reaction was prepared as follows: 28.7 mg of tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Tokyo Chemical Industry) and 3.0733 g of GSH (Nacalai Tesque) were weighed in a 100 mL volumetric flask, 20 mL of 1000 mM potassium phosphate buffer (pH 8.0) was added, and the solution was gently shaken

pylori develops AMO resistance by several main mechanisms: mutations in the PBP gene, -lactamase production, reduced drug penetration to the target site/membrane pore proteins, and mutations in the gene encoding the efflux pump (Lin et al., 2023)

Transl Psychiatry 7 , e1215 (2017)

When there is uneven use of amino acids for protein synthesis in the cytosol the harmonizing process will reestablish concentrations that mirror blood plasma (or media)

WQC, SSL, CC, and JC performed the experiments