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Bottom reading of the microplate provides better measurements since the distance between the cell layer and detector is reduced which minimizes unwanted effects from the cell culture medium

performed the literature search, and all authors drafted, reviewed, and critically revised the final manuscript

Preclinical studies using metabolic assays such as euglycemic clamp techniques indicate that AOD 9604 does not impair insulin-mediated glucose uptake in adipose or peripheral tissues

Acta Neuropathol 125:637650