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Cells were directly (for fresh hepatocytes) or after thawing (for cryopreserved hepatocytes) suspended at a concentration of 1 x 10 6 cells/ml in a seeding medium composed of Williams E medium, supplemented with 10% (vol/vol) FCS, 5 g/ml bovine insulin, 100 IU/ml penicillin, 100 g/ml streptomycin, and 2 mM glutamine

To prevent fragmentation and breakdown of peptides, use 1 to 2.5 ml of solution for reconstitution unless related studies confirm that a higher volume is safe

Hypoxia-inducible factor 1 (HIF-1) is the primary transcription factor regulating angiogenesis (47)

If mitochondria are damaged, they slow down and make less energy

Always allow the vial to come to room temperature before mixing to prevent condensation from forming inside