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PMID: 10707891

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When you are giving a B12 injection subcutaneously, you pinch the fat to make sure youre only penetrating the subcutaneous tissue (or the innermost layer of skin) and pull it away from the muscle, then insert the needle

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PAN-Biotech, Germany) and 0.01% gentamicin (Invitrogen, USA) at 37 C in a humidified atmosphere with 5% CO 2