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Cell suspensions with the targeted cell recovery rate 5000 cells per sample were loaded onto 10 Genomics Single Cell fluidics chips to generate GEMs (Gel Beads-in-emulsion) which include gel beads coated with specific oligonucleotide barcodes to index each cells transcriptome and Unique Molecular Identifiers (UMI) to define individual transcripts
Honda, Y., Kessoku, T., Sumida, Y., Kobayashi, T., Kato, T., Ogawa, Y., Tomeno, W., Imajo, K., Fujita, K., Yoneda, M., Kataoka, K., Taguri, M., Yamanaka, T., Seko, Y., Tanaka, S., Saito, S., Ono, M., Oeda, S., Eguchi, Y., Aoi, W., Sato, K., Itoh, Y., & Nakajima, A
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