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Cells remaining on inside membrane surface in the upper chambers were removed with a cotton swab, whereas the cells on the lower surface were fixed in methanol for 10 min at room temperature, washed with PBS and stained with 0.5% crystal violet and counted as described previously (Gupta et al

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Prevalence of vitamin B12 deficiency among exclusively breast fed term infants in South India. Journal of Tropical Pediatrics 67(1) (2021): fmaa114