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Genomic DNA was extracted using DNeasy mini kit (QIAGEN, Germany), and 100 ng of DNA was used for quantitative PCR with TYLCV-specific primers targeting the TYLCV C1 gene (185 bp) and -actin as the internal control

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Anyone claiming proven human results is going beyond the published evidence

Zaric et al., 2019), and a veritable body of literature has demonstrated the strong association between impaired CS function and AD-associated cognitive decline (Leiteritz et al., 2018

Importantly, the developed procedure was successfully used to determine selected analytes in plasma samples collected from healthy volunteers and the results obtained confirmed the usefulness of the developed method for the analysis of this type of material