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Co-Immunoprecipitation and Immunoblotting Analysis Cells were washed twice with phosphate-buffered saline (PBS), and then lysed in a lysis buffer on ice for 30 min, and then cell lysates were centrifuged at 12,500 rpm for 15 min

If it jumps to 90 BPM or higher, or if you notice irregular rhythm alerts, that warrants a conversation with your provider

doi: 10.1038/s41580-022-00456-z

H.WangQ.et al (2023)

The content is not intended to encourage application, diagnosis, or self-treatment of unlicensed individuals, and should not be used as a substitute for the clinical judgment of a qualified healthcare provider

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